Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.)

The Journal of Environmental studies (JES) 
Vol. (16), 16 pages
doi:.
Author:El-said 'A.H.M.
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Abstract

Fourty-eight species and two species varieties belonging to 19 genera were collected from 50 samples of apple and tomato fruits (25 from each) from Sohag governorate in Egypt on dichloran rose-Benegal chloramphenicol agar medium at 28C. The most common genera were AlternariaAspergillus, Cladosporium, Fusarium and Penicillium. Form the above genera the most prevalent species were Alternaria alternata, A. tenuissima, Aspergillus niger, A. flavus, A. fumigatusCladosporium cladosporioides, Cherbarum and Penicillium oxalicum. Fourty-eight species and two species varieties fungal species screened for their abilities to produce cellulase (C1 enzymeand pectinase enzymes. Six and seven species showed high cellulolytic and pectinolytic activity, while 23 and 17 species were moderately activity for the two enzymes, respectively. The remaining species were low activity in both enzymes. The highest cellulase and pectinase activities were recorded by Aspergillus chevalieri. Maximum production of cellulase enzyme by Achevalieri was obtained after 6 days of incubation at 30C with initial pH 6 in culture medium containing sucrose and peptone as carbon and nitrogen sources, respectively. Regarding to pectinase enzyme the highest pectinase production by A. chevalieri was recorded after 6 days of incubation at 30C with initial pH 8 in culture medium containing pectin and ammonium sulphate as carbon and nitrogen sources, respectively.

Keywords

mycology, postharvest diseases, extracellular enzymes, cellulase, pectinase, apple, tomato, Aspergillus chevalieri.

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