Status of cytoskeleton proteins during initial germination of dark-grown pea (Pisum sativum) seedlings
The Journal of Environmental studies (JES)
Vol.II: 99-107 (2), 9 pages
Original homogenate, supernatant and pellet of 250 xg 27000 xg for 30, 45 and 69 hours germinated Alaska pea seeds (Pisum sativum L. var. Alaska) were tested to investigate the status of cytoskeleton proteins in embryo and its different parts. All samples were treated by cytoskeleton-stabilizing buffer, electrophoresed and blotted on to membranes. Blots probed with antibodies to actin, alpha-tubulin and beta-tubulin, revealed bands with apparent molecular weights of 42 kDa, 46 kDa, and 50 kDa respectively. In all cases, sedimentable tubulin and actin were found maximally in the 27000 xg and 250 xg pellet and less in supernatant of 250 xg and 27000 xg. Amount of actin and tubulin in embryos were a highest in 30 hours germinated Alaska pea seeds and less in 45 hours and much more less in 69 hours. By 96 hours, abundance of cytoskeleton proteins in different fractions of the stems much more high than the prophylls, while in the roots there were very small amounts of all cytoskeleton proteins. Various streptavidin-binding proteins (SBP) and actin like proteins (ALP) were also found in all harvested parts. The present results suggest that cytoskeleton proteins are physiologically important and strongly indicate a crucial role for the differentiation and development of Alaska pea seedlings.
cytoskeleton, actin and tubulin.